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. 2019 Nov 1;12:9053–9058. doi: 10.2147/OTT.S217324

Figure 3.

Figure 3

miR-522-3p promoted GLUT1 expression and increase glucose uptake in OS cells. To investigate the interactions between miR-522-3p and GLUT1, U2OS and MG-63 cells were transfected with miR-522-3p mimic and GLUT1-expression vector. Overexpression of miR-522-3p and GLUT1 was confirmed by qPCR at 24 hrs post-transfection (A). The effects on miR-522-3p overexpression on GLUT1 expression (B) and glucose uptake (C) were analyzed by Western blot, qPCR and glucose uptake assay. The effects of GLUT1 overexpression on miR-522-3p were analyzed by qPCR (D). Mean values of 3 biological replicates were presented. NC, NC miRNA or empty pcDNA3.1 vector-transfected cells; C, untransfected cells; *p<0.05.