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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: J Vis Exp. 2019 Jun 12;(148):10.3791/59496. doi: 10.3791/59496

Figure 1: Schema of proposed steps for isolation of tracheal brush cells.

Figure 1:

The protocol includes 2 major steps: after dissection, the whole trachea is incubated in a high-dose dispase solution to separate the epithelium; this is followed by digestion of the epithelial sheet with papain in calcium containing Tyrode buffer (Tyrode I) and antibody staining.