Table 2: Rh59 (UL35) deletion reduces dissemination of 68–1 RhCMV vectors.
Three naturally RhCMV-infected RMs (T2–1, T2–2, T2–3) were co-inoculated with 107 PFU each of 68–1/SIVrtni (left arm) and ΔRh59/SIVgag (right arm). One RM was necropsied at 14, 21, or 28 dpi and viral genome copy numbers per 107 cell equivalents in the indicated tissues were determined using ultra-sensitive nested qPCR specific for SIVrtni (68–1) or SIVgag (ΔRh59).
| Table 2 | Super infection: 68–1 vs. ∆Rh59 | |||||
|---|---|---|---|---|---|---|
| RM T2–1 | RM T2–2 | RM T2–3 | ||||
| Tissue type | 14 dpi | 21 dpi | 28 dpi | |||
| 68–1/SIVrtni | ∆Rh59/SIVgag | 68–1/SIVrtni | ∆Rh59/SIVgag | 68–1/SIVrtni | ∆Rh59/SIVgag | |
| Skin Inj site-right (∆Rh59) | 12 | <1 | 193 | <1 | 36,110 | 7 |
| Skin Inj site-left (68–1) | 541 | <1 | 23,793 | <1 | 4,480,601 | <1 |
| Axillary LN | 3 | <1 | 13 | <1 | 2,515 | 7 |
| Peripheral LN | <1 | <1 | <1 | <1 | 18 | <1 |
| Liver/Gallbladder | <1 | <1 | <1 | <1 | 2 | <1 |
| Heart/Lung/Kidney/BAL | 2,933 | <1 | 3 | <1 | 6 | <1 |
| Bone marrow/Spleen/Tonsil | 6 | <1 | <1 | <1 | 8 | <1 |
| Neuro/Endocrine | 3 | <1 | <1 | <1 | 4 | 1 |
| Genitourinary tract | <1 | <1 | 3 | <1 | 9 | <1 |
| Salivary gland | 2 | <1 | 4 | <1 | 3 | <1 |
Normalized to 1×107 cell equivalents