micropillar chip; herringebone chip |
5–1281 CTCs/mL with 1 mL/h, detection rate 65%; 63 CTCs/mL with 1.2 mL/h, detection rate 91.8 ± 5% for PC3; 50.3 CTCs/mL with 8 mL/h, detection rate 98% SKBR3 |
M. Toner15,35–37
|
micropillar chip |
30 CTCs/mL with 1 mL/h, detection rate 97 ± 3% |
B.J. Kirby38
|
sinusoidal chip |
53 CTCs/mL with 15 mL/h, detection rates are 98% MCF-7 and 82% MDA-MB-231 |
S. A Soper39–42
|
microchannel array |
10 CTCs/mL with 36 μL/min, recover rate >95%; using additional grooved surface can achieve over 90% capture rate with >84% purity; throughput 3.6 mL/h |
Z. Fan43,44
|
μHall: antibody labeled magnetic nanoparticles |
recovery rate of MDA-MB-468 99%; purity 100%; throughput 3.25 mL/h; analyzing ovarian 7.6 CTCs/mL |
H. Lee45
|
CellSearch system |
positive selection to enrich tumor cells from whole blood; the enriched and stained cells are scanned and imaged on CellTrackersAnalyzer II |
8, 46–49
|
microfilter |
0–12.5 CTCs/mL with 90 mL/h, detection rate 90% |
M. Toner35,50
|
portable filter |
MCF-7, SK-BR-3:5 CTCs/mL at 0.5 psi constant pressure, detection rate >90% |
R.J. Cote,51
|
microfilters |
1–3 CTCs/mL with 2 mL/h, detection rate enhanced with antibody coating: 95% MCF-7; 97% MDA-MB-231 |
C.T. Lim,52
|
microsieve filter with uniform pore structure |
undiluted whole blood with flow rate <2 mL/min; MCF-7 recover rate >80%; accurately detected CTC from eight patients |
M. Li53
|
3D parylene-C micronlter |
selection by both size and deformability; collect >85% viable spiked MCF-7 cells |
Y. Tai28
|
suspended microchannel resonator |
flow rate 45 μL/h; analyze 500 cells/h to reach 73% unhealthy cells; healthy blood cells passage time <10 ms; CTC passage time >10 ms |
S. Manalis54–56
|
lab-on-a-disc: centrifugal microfluidic size selection |
MCF-7 61% capture rate; have the potential for high throughput and easy detection by directly mL; throughput >3 mL/min; sensitivity 95.9 ± 3.1% recovery rate; selectivity > 2.5 log WBCs depletion |
Y. Cho57,58
|
vortex |
capture CTC by fluidic vortex without having CTCs experience deformation; throughput 7.5 mL whole blood in 20 min; about 60–90 min on the system with no sample prep; no staining or any kind of affinity capture of the cells is required; efficiency of capture 60–80% |
D. Di Carlo31,32,59–61
|