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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Cell Rep. 2019 Jul 23;28(4):1003–1014.e3. doi: 10.1016/j.celrep.2019.06.077

Figure 2. Differential Responses of ChIs to Thalamic and Cortical Stimuli.

Figure 2.

(A) Representative cell-attached recording of a ChI illustrating high average firing response rate due to thalamic burst stimulus (5 pulses, 50 Hz, 470 nm). Inset: zoom of stimulation period.

(B) Representative cell-attached recording of a ChI illustrating low average firing response due to cortical burst stimulus (5 pulses, 50 Hz, 470 nm). Inset: zoom of stimulation period.

(C and D) Peristimulus time histograms of ChI firing frequency during stimulation of either thalamic (C) or cortical (D) input. Error indicates ± SEM.

(E) Summary comparison of baseline ChI action potential probability during each trial with the probability during the 100-ms thalamic stimulus period (n = 13; p < 0.001, Wilcoxon matched pair signed rank), indicating a strong increase in ChI activity following thalamic stimulation, whereas cortical inputs selectively activate a subset of ChIs during the same stimulus (thalamus: n = 13; cortex: n = 29; p < 0.001, Mann-Whitney).

(F) Summary comparison of baseline ChI action potential probability during each trial with the probability during the 100-ms cortical stimulus period (n = 29; p > 0.05, Wilcoxon matched pair signed rank). Although ChIs are overall unresponsive to cortical excitation, some neurons increase their activity due to the stimulus.

(G) Distribution of spike latencies for responding ChIs following thalamic or cortical stimulation (thalamus: n = 11; cortex: n = 10; p < 0.01, Mann-Whitney). **p < 0.01, ***p < 0.001. Error bars indicate ± SEM.