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. 2019 Nov 1;14:8543–8560. doi: 10.2147/IJN.S219820

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© 2019 Zhong et al.

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In vitro cytotoxicity and hemolysis assay of nanoparticles.

Notes: (A) COLO205 colorectal cancer cells were treated with PLGA-PTX over a time course, and this was followed by cell viability measurement using the CCK-8 assay. (B) COLO205 cells were treated with free PTX or encapsulated PLGA-PTX combined with or without iRGD for 72 h. (C) Cytotoxicity of PTX or PLGA-PTX against various colorectal cancer cells. (D) Hemolysis assay. DI water and NS were used as the positive (100% hemolysis) and negative control, respectively. The appearance of hemolysis (left panel) and percentage of hemolysis (right panel) were assessed for each group.

Abbreviations: PLGA, poly lactic-co-glycolic acid; PTX, paclitaxel; PLGA-PTX, paclitaxel-loaded PLGA; DI water, deionized water; NS, normal saline.