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. 2019 Nov 1;14:8543–8560. doi: 10.2147/IJN.S219820

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© 2019 Zhong et al.

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Tumor vasculature distribution and expression of the iRGD receptor.

Notes: LS174T tumor frozen sections were prepared for immunofluorescence staining and imaging. (A) Blood vessel distributions were stained with anti-CD31 antibody (red). (B) The expression of iRGD receptors, including integrin αv, integrin α5, and neuropilin-1, were assayed by staining with anti-receptor antibodies (red). Tumor blood vessels (green) and nuclei (blue) were co-stained with anti-CD31 antibody and DAPI. Scale bar, 100 µm.

Abbreviations: CD31, cluster of differentiation 31; DAPI, 4,6-diamidino-2-phenylindole.