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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Nat Microbiol. 2019 Jul 22;4(11):1851–1861. doi: 10.1038/s41564-019-0498-2

Fig. 1 |. Peritoneal treatment with 12,13-diHOME exacerbates lung inflammation in mice challenged with CRA.

Fig. 1 |

a, Haematoxylin and eosin-stained bronchioles and blood vessels from the lungs of mice treated with vehicle (10% DMSO) and challenged with PBS or CRA, or treated with 30 mgkg−1 12,13-diHOME (solubilized in 10% DMSO) and challenged with CRA. Scale bars, 50 μm. Representative of four experimental repeats. b, Peritoneal 12,13-diHOME treatment (n = 22 mice) increases serum IgE compared with vehicle-treated CRA-challenged mice (n = 19 mice; linear mixed effects (LME), P= 0.019) or vehicle-treated, PBS-challenged mice (n = 14 mice; LME, P= 0.0088). c,d, 12,13-diHOME treatment (n = 19 mice) increases the number of infiltrating cells surrounding the bronchioles (c) and veins (d) of CRA-challenged mice compared with mice treated with vehicle and challenged with PBS (n = 10 mice; LME, P = 0.0202 (bronchial), P = 0.000122 (venous)) or mice treated with vehicle and challenged with CRA (n = 18 mice; LME, P=0.0449 (bronchial), P = 0.00127 (venous)). e, Peritoneal 12,13-diHOME treatment increases the number of lung-resident T cells (CD3+) in CRA-challenged mice (n = 8 mice) compared with mice treated with vehicle and challenged with PBS (n = 10 mice; LME, P = 4.69×10−6) or mice treated with vehicle and challenged with CRA (n = 10 mice; LME, P = 0.016). f, 12,13-diHOME treatment of mice challenged with CRA (n = 8 mice) increases the expression of IL1B compared with expression in mice treated with vehicle and challenged with PBS (n = 9 mice; LME, P=0.0010) or mice treated with vehicle and challenged with CRA (n = 9 mice; LME, P = 0.030). g, 12,13-diHOME treatment of mice challenged with CRA (n = 18 mice) decreases the number of Treg cells compared with the number in mice treated with vehicle and challenged with CRA (n = 18 mice; LME, P= 0.031). h, Peritoneal treatment of mice with 12,13-diHOME significantly increases the concentration of 12,13-diHOME in the lungs (n = 7 mice; LME, P = 0.0048) at 3h after delivery. Unique symbols (triangles, squares, diamonds, circles, pluses, asterisks and crosses) represent mice from independent repeats. All data were plotted using boxplots from ggplot2, which displays the median, the 25th and 75th percentiles, and the smallest and largest values within 1.5× the interquartile range (whiskers). See also Supplementary Fig. 1.