Table 2.

Experience of using antigen-coupled cell based tolerance in rodent models of disease.

Citation	Disease Model	Antigen-Specific Therapy	Treatment Regimen	Estimated Antigen per Dose	Results
18,22,46,47	EAE	Myelin peptide/proteins	50 × 106 antigen-coupled splenocytes (coupled with 0.5 mg/mL peptide in the presence of ECDI).	24-55 μg/dose	Treatment prevented or reversed disease depending on when treatment was initiated.
6	TID	Full length bovine insulin	NOD mice were injected i.v. with 5 × 107 insulin coupled splenocytes at numerous time points. (coupled with 0.5 mg/mL peptide in the presence of ECDI).	Not defined	Demonstration of disease prevention or remission, inhibition of pathogenic T cell proliferation, decreased cytokine production, and induction of anergy.
48	TID	1040-p31 peptide	NOD.BDC2.5.Thy1.1 TCR Tg+ cells transfer into NOD/scid mice; mice received 50 × 106 antigen-coupled splenocytes (coupled with 0.5 mg/mL peptide in the presence of ECDI).	Not defined	Demonstration of disease prevention or remission, inhibition of pathogenic T cell proliferation, decreased cytokine production, and induction of anergy.
23	Islet Transplant	ECDI fixed allogeneic splenocytes	1 × 108 ECDI-treated allogeneic splenocytes were transferred into streptozotocin-treated mice that received allogeneic beta-islet cells under the kidney capsule.	Not defined	Demonstration of indefinite survival of allogeneic islet grafts in the absence of immunosuppression
49	EAT	Thyroglobulin-coupled spleen cells	Suppression of experimental autoimmune thyroiditis in guinea pigs.	133 μg/dose	Prevention of Thyroiditis in all pre-treated animals
50	Uveitis	S-antigen (S-Ag) and interphotoreceptor retinoid binding protein (IRBP)	Injection of S-Ag or IRBP coupled to spleen cells 5 days prior to immunization with native S-Ag or IRBP, respectively.	Protein homogenate, unknown concentration of antigens	Prevention of Uveitis in 6/8 rats
51	Neuritis	Myelin P253-78	Lewis rats receive 100 μg of SP-26/CFA; treated with 20 μg/1 × 107 splenocytes and each rat received 50 × 106 coupled cells.	Not defined	Prevention of neuritis