Skip to main content
. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: J Immunol. 2018 Feb 5;200(6):2013–2024. doi: 10.4049/jimmunol.1700326

FIGURE 6.

FIGURE 6.

ILDR2-hFc regulates immune human T cell responses by downregulating Th1 and Th17 and upregulating Th2 differentiation. Naive human CD4+ T cells were isolated from total PBMCs from four healthy human donors and cocultured with autologous irradiated PBMCs. T cells were then activated with anti-CD3 (0.5 μg/ml) and soluble anti-CD28 (0.5 μg/ml) in the presence of Th1 (A and B), Th17 (C), or Th2 (D–F) differentiation-promoting conditions, as detailed in Materials and Methods. ILDR2-hFc or control Ig (hIgG1) was added at 10 μg/ml. Supernatants were collected at 96 h, and cytokine levels were evaluated in duplicate. One representative experiment of two is presented. ***p < 0.001 versus control Ig.