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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: J Cell Biochem. 2018 Mar 25;119(7):6016–6023. doi: 10.1002/jcb.26798

Figure 4. Cortisol does not affect the stability of Hey1, Hey2 or HeyL mRNA.

Figure 4.

Primary osteoblast-enriched cells from the parietal bones of 3 to 5 day old wild-type C57BL/6J mice were seeded on DLL1. Cells were allowed to recover for 24 h and subsequently cultured for 24 h in the absence of serum and then exposed to vehicle or cortisol 1 μM for 6 h. Cells were transcriptionally arrested with actinomycin D (Time 0) and harvested at the indicated times. Total RNA was extracted and mRNA levels quantified by qRT-PCR. Data are expressed as percent of Hey1, Hey2 and HeyL mRNA levels corrected for Rpl38 copy number prior to exposure to actinomycin D, plotted versus time. Values are means ± SD; n = 4. No differences between cortisol and control slopes of decay by ANCOVA.