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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: J Cell Biochem. 2018 Mar 25;119(7):6016–6023. doi: 10.1002/jcb.26798

Table 1. Primers for qRT-PCR.

Forward (Fwd) and reverse (Rev) primers used for detection of mRNA and hnRNA by qRT-PCR. GenBank accession numbers indicate transcript variants with homologous sequences to primers.

Gene RNA
species
Strand Primer Sequence 5’ to 3’ GenBank
Accession Numbers
Hey1 mRNA Fwd
Rev
ATCTCAACAACTACGCATCCCAGC
GTGTGGGTGATGTCCGAAGG
NM_010423
hnRNA Fwd
Rev
CCCAACGACATCGTCCCAGGTTT
GGGTACTCACGCCTCTCCGTCTTT
Hey2 mRNA Fwd
Rev
AGCGAGAACAATTACCCTGGGCAC
GGTAGTTGTCGGTGAATTGGACCT
NM_013904
hnRNA Fwd
Rev
CGCCCTTGTGAGGAAACG
CGCTCACCCAGGGTAATTG
HeyL mRNA Fwd
Rev
CAGTAGCCTTTCTGAATTGCGAC
CCCAGCACAACTCCTCCCTA
NM_013905
hnRNA Fwd
Rev
GAGAACGATCTTAGGTAAGACAGG
AACTTGAGTGTGCTGGACTT
Rpl38 mRNA Fwd
Rev
AGAACAAGGATAATGTGAAGTTCAAGGTTC

CTGCTTCAGCTTCTCTGCCCTTT
NM_001048057
NM_023372
NM_001048058
Tsc22d3 mRNA Fwd
Rev
GCGTCATCTCATGTGGAGAACTT
CTCTTGGCACCAGCTATGTTAGG
NM_010286
NM_001077364