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. 2019 Aug 23;30(11):2140–2157. doi: 10.1681/ASN.2019030236

Figure 3.

Figure 3.

MPO/BAY DCs attenuate anti-MPO GN. MPO/BAY DCs (n=6) or saline (n=7) were administered to mice with established anti-MPO immunity (day 14) and GN assessed on day 26 (experimental design shown in Figure 2A). (A) The proportion of abnormal glomeruli. (B) Representative photomicrographs showing glomerular injury in mice treated with vehicle or BAY DCs. Formalin-fixed kidney sections were stained with periodic acid–Schiff (hematoxylin counterstain). Original magnification, ×400. (C) Proteinuria (in the last 24 hours). (D) Glomerular CD4 T cells, CD8 T cells, neutrophils, and macrophages. (E) Representative photomicrographs showing glomerular CD4 cells, CD8 cells, and neutrophils. Leukocytes (brown) were assessed by immunohistochemistry (hematoxylin counterstain). Original magnification, ×400. (F and G) Collagenase/DNase-digested kidneys were stained for macrophages (CD45+F4–80+) and M1 (iNOS, IL-12p40) and M2 markers (MR, IL-10), and analyzed by flow cytometry. (F) The proportion of renal macrophages expressing iNOS, IL-12p40, MR, or IL-10. (G) Representative flow cytometry plots showing the proportion of IL-10+ renal macrophages. Data are presented as scatter plots with the mean±SEM. *P<0.05, **P<0.01. c/gcs, cells per glomerular cross section.