Figure 2.

Activation of Hedgehog pathway by overexpressing Smoothened-M2 has no effect on cyst progression in autosomal dominant polycystic kidney disease models. (A) Images of representative kidneys from the perinatal collecting duct knockout model with the indicated genotypes at postnatal day 24. (B) Aggregate data of the kidney weight-to-body weight ratio, cystic index, and BUN from the indicated number of mice. The color blocks in A correspond to the data in B. Comparison of two datasets using the Mann–Whitney test, presented as mean±SEM, showed no significant differences in kidney weight-to-body weight ratio, cystic index, or BUN. The color blocks in A correspond to the data in B. (C) Images of representative kidneys of adult inducible whole-nephron knockouts with the indicated genotypes at 18 weeks. (D) Aggregate data of the kidney weight-to-body weight ratio, cystic index, and BUN from the indicated number of mice. The color blocks in C correspond to the data in D. Comparison of two datasets using the Mann–Whitney test, presented as mean±SEM, showed no significant differences between the two models. Scale bars, 2 mm in A and C.