Figure 3.

Inactivation of Gli2 and Gli3 does not affect cyst formation in a Pkd1 model. (A) Representative images of kidneys with the indicated genotypes. Doxycycline was administered from postnatal day 0 (P0) to P14, and kidneys were analyzed at P14. (B) Aggregate data of the kidney weight-to-body weight ratio, cystic index, BUN, and serum creatinine concentrations of different genotypes. The color blocks in A correspond to the data in B. The comparison used one-way ANOVA followed by Tukey multiple comparison test and is presented as mean±SEM. To facilitate the breeding of the multiple alleles and the use of littermates as controls, we used mice heterozygous for Gli2^fl or Gli3^fl as controls and compared with Gli2^fl/fl and/or Gli3 ^fl/fl. Scale bar, 2 mm. **P=0.002 for the comparison of each of the four cystic models with the wild-type control; ****P<0.001 for the comparison of each of the four cystic models with the wild-type control.