Figure 1.

Short-term Npt2a inhibition causes a dose-dependent increase in urinary phosphate, calcium, sodium, and chloride excretion. Response to acute oral (p.o.) application of Npt2a-I (0.3–300 mg/kg via oral gavage) or vehicle in short-term (3 hour) metabolic cage experiments. (A) Dose-dependent increase in urinary phosphate excretion in absolute terms (50% effective dose [ED₅₀] of 21 mg/kg), and (B) when corrected by urinary creatinine. (C and D) Similar effects were observed for calcium, (E and F) sodium, and (G and H) chloride. Data are expressed as mean±SEM. n=6–14 for 0.3–100 mg/kg; n=3 for 300 mg/kg.