Figure 4.

Npt2a inhibition affects Npt2a but not Npt2c. Western blot analysis of Npt2a and Npt2c abundances in membrane fractions and total homogenates of kidneys from mice treated (30 mg/kg) with vehicle (V) or Npt2a inhibitor (I). (A) Npt2a membrane abundance was significantly increased 3 hours after Npt2a-I treatment and not different from vehicle treatment after 24 hours. (B) Total Npt2a abundance was unaffected after 3 hours and significantly reduced after 24 hours. In contrast, no effect was observed at any time point in (C) membrane fractions or (D) total homogenate Npt2c abundance. Data were analyzed by unpaired t test and are expressed as mean±SEM, n=5–6. *P<0.05 versus vehicle.