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. 2018 Sep 6;24(7):430–438. doi: 10.1177/1753425918796619

Figure 2.

Figure 2.

NO and iNOS production in RAW264.7 cells infected with SeV 4C(–). (a–d) Cells were mock-infected or infected with SeV strains at MOI 5 (a, c, d), or as indicated (b). Culture media were collected 24 h postinfection (a, b) or at the indicated time points (c) and assayed for nitrite. Cells were also harvested at the indicated time points and immunoblotted with anti-iNOS (d). (e) Cells were transfected with pCA7HA vector with (CSeV) or without (Empty) C protein, along with pRL-TK or piNOS-Luc, a firefly luciferase reporter plasmid driven by the iNOS promoter. At 24 h posttransfection, cells were mock-infected or infected with SeV 4C(–) at MOI 5, harvested eight h thereafter, and assayed by a dual-luciferase assay system to evaluate activation of the iNOS promoter. *P < 0.01 vs infection with wtSeV (a), vs mock infection (b,c), or vs pCA7HA empty vector.iNOS: inducible NO synthase; SeV: Sendai virus; TK: thymidine kinase; wt: wild type.