Figure 1.

Isolation of saliva plasma phospholipid transfer protein (PLTP) by heparin affinity column run with a HPLC technique. (a) Saliva was collected from six apparently healthy laboratory staff members and pooled. Then 1 ml of saliva was applied on Heparin-Sepharose CL-6B-GE column and the elution of PLTP was followed by absorbance (280 nm) and PLTP activity. Fractions 1-2, 3-4, and 11-13 were combined separately representing non-bound ‘through’, non-bound ‘wash’ and bound material, respectively. (b) Western blot of the combined fractions with PLTP activity after heparin affinity chromatography. The SDS-PAGE was run under reducing and non-reducing conditions. Volumes applied on the gel: saliva 5 µl, non-bound ‘through’ 10 µl, non-bound ‘wash’ 30 µl, and bound 40 µl. A representative elution curve and Western blot from three experiments using the same saliva pool is shown.