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TLRs regulate IFN-γ and IL-4 secretion. Single lung cell suspensions of normal and infected mice were prepared and then cultured in the presence of different ligands plus anti-CD3 mAb. The culture supernatants were collected after 72 h of incubation for detection of IFN-γ and IL-4 by ELISA. The data are representative of four experiments, each with three or four replicates per group (*P < 0.05; the error bars indicate SD).
