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. 2018 Nov 21;25(1):13–21. doi: 10.1177/1753425918812646

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© The Author(s) 2018

Creative Commons CC BY: This article is distributed under the terms of the Creative Commons Attribution 4.0 License (http://www.creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 3. — Gene expression analysis of osteoclast differentiation induced by E. faecalis LTA in osteoclast precursors. WT BMMs were treated for 6 d in the presence of 40 ng/ml M-CSF with the three E. faecalis LTAs, RANKL, E. faecalis LTAs plus RANKL, or pre-treated with RANKL for 3 d prior to treatment with E. faecalis LTAs for an additional 3 d, respectively. The gene expression levels of (a) c-Fos, (b) NFATc1, (c) Notch1 and (d) RBP-J were assayed using real-time PCR. The numbers of abscissa represent the same treatment groups as those in Figure 2. The mean and SD are shown. *P < 0.05 was considered statistically significant compared with untreated cells or RANKL-only treated cells.