Table 1.
The effect of rhIL-10 on the MyD88-dependent and -independent cytokines.
| IL-1β | IL-6 | IL-8 | TNF-α | IP-10 | RANTES | |
|---|---|---|---|---|---|---|
| Vehicle (no bacteria) | 0 | 0 | 0 | 0 | 515 | 773 |
|
|
(0–0) |
(0–0) |
(0–0) |
(0–179) |
(274–855) |
(405–6943) |
| N. meningitidis wt | 3790 (2666–6654) | 7945 (6317–11,481) | 1545 (1128–2350) | 25,929 (5377–34,772) | 1769 (1152–5555) | 5601 (4615–7871) |
| N. meningitidis wt + rhIL-10 (25 ng/ml) | 61 (36–172) | 1011 (862–2029) | 256 (173–355) | 1060 (332–2773) | 1031 (617–2040) | 5062 (1388–6900) |
| Fold reduction |
62.1*
|
7.9*
|
6.0*
|
24.5*
|
1.7*
|
1.1
ns
|
| N. meningitidis lpxL1 mutant | 407 (245–2015) | 3214 (1947–8956) | 1015 (853–3053) | 5766 (1155–15,816) | 583 (482–1169) | 3443 (776–7576) |
| N. meningitidis lpxL1 mutant + rhIL-10 (25 ng/ml) | 6 (0–30) | 89 (37–513) | 119 (63–246) | 68 (23–567) | 663 (388–1425) | 1171 (1057–6718) |
| Fold reduction | 67.8* | 36.1* | 8.5* | 84.8* | 0.9 ns | 2.9 ns |
Whole blood from six donors was incubated for 4 h with N. meningitidis wt (108/ml), N. meningitidis lpxL1mutant (108/ml) or vehicle, in the absence or presence of recombinant IL-10 (rhIL-10), plasma collected, and measurements of cytokines performed. The concentrations [median (range)] (pg/ml) of the cytokines, and fold reduction between ± rhIL-10 are shown. *Indicates significant differences in cytokine levels with and without rhIL-10 in whole blood incubated with either the N. meningitidis wt, or the N. meningitidis lpxL1 mutant (P < 0.05). ns: non-significant differences.