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. 2018 May 31;24(5):323–331. doi: 10.1177/1753425918777598

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 1. — The Aa following Pam2Cys determines the cross-priming activity in bone marrow–derived DC (BMDC). (a) and (b) BMDCs were stimulated with Pam2LPs in the presence of OVA. Carboxyfluorescein diacetate succinimidyl ester (CFSE)-labelled OT-I cells were co-cultured with the BMDCs. After 60 h, the degree of OT-I proliferation was assessed by flow cytometry. (a) OT-I cells were gated as CD8α⁺ TCR Vβ5.1,5.2⁺ population. The histograms of representative samples are shown. (b) The result including all samples is shown. The results are representative of two independent experiments.