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. 2018 May 31;24(5):323–331. doi: 10.1177/1753425918777598

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© The Author(s) 2018

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — Pam2-13s is not directly recognized by OT-I cells. (a) Tlr2 gene expression level on OT-I cells and CD8α⁺ DCs isolated from OT-I mouse were analysed by real-time PCR. Error bars show ±SD. (b) Splenocytes were harvested from OT-I mouse and TLR2. TLR6 expression levels on OT-I cells (CD8α⁺ CD3⁺ population) and CD8α⁺ DCs (CD8α⁺ CD11c^hi population) were analysed by flow cytometry. Shaded and open histograms indicate isotype control and TLR2, TLR6 staining, respectively. (c) CFSE-labelled OT-I cells were cultured singularly in the presence or absence of anti-CD28 and anti-CD3 Abs. PBS, Pam2-13 and Pam2-13s were added to the culture media. After 60 h, the degree of OT-I proliferation was assessed. The histograms of each sample are shown.