Figure 5. qPCR of multiple loci confirms the presence of the cancer lineage in M. trossulus and M. edulis from multiple populations.

Allele-specific qPCR was conducted to determine the fraction of cancer-associated alleles at the four loci: allele H at EF1α, allele RM and KNS at H4, allele C and D at mtCR, and allele B at mtCOI (see Figure 5—source data 1). Boxes show whether the cancer-specific allele is undetectable or detectible, with a heatmap showing the fraction of the total alleles at each locus that correspond to the specific allele (the quantity of amplification with an allele-specific qPCR reaction divided by the quantity amplified with ‘universal’ Mytilus primers). Each individual mussel is marked with its ID, species, and location, and each animal that was diagnosed as containing disseminated neoplasia is marked ‘Cancer’.