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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Cell Rep. 2019 Sep 10;28(11):2739–2747.e4. doi: 10.1016/j.celrep.2019.08.009

Figure 1. Fibers from the vlPAG Are Distributed throughout the VTA.

Figure 1.

(A) Schematic of AAV2-hSyn-ChR2-mCherry injections into bilateral vlPAG.

(B) Sample horizontal slice with bilateral virus injection sites into the vlPAG marked in magenta (Aq, aqueduct; DR, dorsal raphe; scale bar, 500 μm).

(C) Cell bodies in the PAG with mCherry expression (magenta; scale bar, 250 μm).

(D) Sample horizontal brain slice with mCherry-fiber expression and TH immunocytochemical labeling, acquired and stitched with 2D slide scan in MBF Stereoinvestigator (green; scale bar, 250 μm).

(E and F) Confocal images at high magnification of TH(+) neurons (green) in the VTA surrounded by mCherry bouton-like profiles (E) and axon fibers (F) from the vlPAG (magenta; scale bar, 20 μm).