KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| C-Fos antibody | Cell Signaling Technology | Cat#4384; RRID: AB_2106617 |
| TH antibody | Millipore | Cat#AB152; RRID: AB_390204 |
| NeuN antibody | EMD Millipore | Cat#MAB377; RRID: AB_2298772 |
| Cy5 Goat anti-rabbit IgG | Jackson Immuno Research | Cat#111-175-144; RRID: AB_2338013 |
| Alexafluor 594 Donkey anti-rabbit IgG | Jackson Immuno Research | Cat#711-585-152; RRID: AB_2340621 |
| Cy5 Goat anti-mouse IgG | Jackson Immuno Research | Cat#115-175-146; RRID: AB_2338713 |
| FITC streptavidin | Jackson Immuno Research | Cat#016-010-084; RRID: AB_2337236 |
| Bacterial and Virus Strains | ||
| AAV2-hSyn-hChR2(H134R)-mCherry | University of North Carolina Vector Core, stock constructs from the laboratory of K. Deisseroth at Stanford University | n/a |
| AAV2-hSyn-mCherry | University of North Carolina Vector Core, stock constructs from the laboratory of K. Deisseroth at Stanford University | n/a |
| AAV2-hSyn-eNpHR3.0-mCherry | University of North Carolina Vector Core, stock constructs from the laboratory of K. Deisseroth at Stanford University | n/a |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Fluoro-Gold | Biotium | Cat#80023 CAS 223769-64-0 |
| Biocytin | Sigma-Aldrich | Cat#B4261 CAS 576-19-2 |
| Histamine | Sigma-Aldrich | Cat#H7250 CAS 56-92-8 |
| Serotonin | Sigma-Aldrich | Cat#H9523 CAS 153-98-0 |
| Bradykinin | Sigma-Aldrich | Cat#B3259 CAS 6846-03-3 |
| Prostaglandin E2 | Millipore | Cat#538904 CAS 363-24-6 |
| 4-Aminopyridine | Sigma-Aldrich | Cat#275875 CAS 504-24-5 |
| Tetrodotoxin | Tocris | Cat#1078 CAS 4368-28-9 |
| Gabazine (SR 95531) | Tocris | Cat#1262 CAS 104104-50-9 |
| DNQX | Tocris | Cat#0189 CAS 2379-57-9 |
| Paraformaldehyde | Sigma-Aldrich | Cat#158127 CAS 30525-89-4 |
| Vectashield | Vector Labs | Cat#H-1000 |
| AP-5 | Tocris | Cat#0106 CAS 79055-68-8 |
| Deposited Data | ||
| A midbrain circuit that mediates headache aversiveness | Mendeley Data | https://doi.org/10.17632/yhtnjnf2jf.1 |
| Experimental Models: Organisms/Strains | ||
| Model organism: Sprague Dawley rat | Charles River Laboratories | n/a |
| Software and Algorithms | ||
| Igor Pro 6 | WaveMetrics | RRID: SCR_000325 |
| Viewer II software | Biobserve | RRID: SCR_014337 |
| Stereoinvestigator | MBF | n/a |
| SPSS 25 | IBM | RRID: SCR_002865 |
| Other | ||
| Nanoject II | Drummond Scientific | Cat#3-000-204 |
| PixyCam | Charmed Labs | n/a |
| Arduino Uno | SparkFun Electronics | Cat#DEV-11021 |