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. 2019 Oct 2;55(6):1313–1323. doi: 10.3892/ijo.2019.4890

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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NELFE promoted the malignant biological behavior of PC cells. (A) The mRNA expression of NELFE was decreased following transfection of the NELFE-shRNA vector in PaCa-2 and PANC-1 cells. (B) The protein expression of NELFE was decreased following transfection of the NELFE-shRNA vector in PaCa-2 and PANC-1 cells. (C) MTT assay demonstrated that decreased NELFE suppressed PC cell growth. (D) The decreased NELFE inhibited clone formation of PC cells. (E) Transwell assay revealed that knockdown of NELFE inhibited PC cell invasion and migration. The invaded cells were quantified by counting the cells in 10 randomly selected fields (×200 magnification). All experiments were repeated in triplicate. ^*P<0.05, ^**P<0.01. NELFE, negative elongation factor E; PC, pancreatic cancer; shRNA, short hairpin RNA; NC, negative control.