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. 2019 Oct 2;55(6):1313–1323. doi: 10.3892/ijo.2019.4890

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Copyright: © Han et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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NELFE promoted EMT and Wnt/β-catenin signaling way in PC. (A) Western blot analysis of EMT-associated protein and β-catenin expression after the downregulation of NELFE in PC cells. GAPDH was used as an internal control. (B) Downregulation of NELFE in PC cells reduced the nuclear accumulation of β-catenin. (C) The dual luciferase reporter assay showed that decreased NELFE inhibited the transactivation of the TCF reporter in PC cells. (D) Reverse transcription-quantitative PCR assay revealed that the expression of the Wnt/β-catenin downstream genes (c-Myc, Survivin and cyclinD1) was decreased due to the knockdown of NFLFE in PC cells. All experiments were repeated three times. ^*P<0.05, ^**P<0.01 vs NC group. NELFE, negative elongation factor E; EMT, epithelial-to-mesenchymal transition; PC, pancreatic cancer; shRNA, short hairpin RNA; NC, negative control.