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. 2019 Oct 2;55(6):1313–1323. doi: 10.3892/ijo.2019.4890

Figure 4.

Figure 4

NDRG2 is an essential downstream effect of NELFE in PC. (A) NDRG2 expression was measured by western blotting in PC cells with NELFE knockdown. (B) The correlation between NELFE and NDRG2 mRNA expression in 120 samples from patients with PC was analyzed via person correlation analysis (R=−0.776). (C) Western blot analysis of associated protein and β-catenin expression following the downregulation of NDRG2 in PC cells with decreased NELFE. (D) Decreased NDRG2 enhanced the nuclear accumulation of β-catenin in PC cells with decreased NELFE. (E) MTT assay showed downregulation of NDRG2 rescued the function of sh-NELFE on PC cells proliferation. (F) The downregulation of NDRG2 rescued the function of decreased NELFE on PC cells colony formation ability. (G) Knockdown of NDRG2 rescued the inhibition function of decreased NELFE on PC cells migration and invasion. The PC cells with NELFE-NDRG2 double knockdown cells were used in the panels of C-G. All experiments were repeated in triplicate. *P<0.05, **P<0.01 vs. NC group. NDRG2, N-Myc downstream-regulated gene 2; NELFE, negative elongation factor E; EMT, epithelial-to-mesenchymal transition; PC, pancreatic cancer; shRNA, short hairpin RNA; NC, negative control.