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. 2019 Sep 30;55(6):1296–1312. doi: 10.3892/ijo.2019.4887

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Copyright: © Ye et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Anisomycin inhibits HuOCSC invasion and angiogenesis in vitro. (A) A Transwell invasion assay was used to investigate the invasive properties of CD44⁺/CD133⁺ HuOCSCs following anisomycin treatment (n=4). Magnification, ×100. (B) Representative images and (C) quantification of the ability of HUVECs to form 3-dimensional tubular structures in Matrigel following anisomycin treatment (n=4). Magnification, ×100. (D) Quantification and (E) representative images of the growth and development of intersegmental vessels and dorsal longitudinal anastomotic vessels in zebrafish embryos following anisomycin treatment (n=30). Magnification, ×40. ^**P<0.01 vs. non-treated. HuOCSCs, human ovarian cancer stem cells; GFP, green fluorescent protein.