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. 2019 Sep 30;55(6):1296–1312. doi: 10.3892/ijo.2019.4887

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Copyright: © Ye et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Anisomycin inhibits the in vivo activity of CD44⁺/CD133⁺ HuOCSCs. (A) Tumours formed by anisomycin-pre-treated and DMSO-pretreated HuOCSCs in nude mice. (B) Volume of tumours throughout the experiment. (C) Final tumour weights at 35 weeks. (D) H&E staining histopathology analysis of the xenografted tumours. Blood vessels are indicated by blue arrows. Magnification, ×200. (E) Immunofluorescence staining of the density of CD31/Flt-1 and FVIII/CD146 double-positive cells in the xenografted tumours. Magnification, ×200. ^**P<0.01 vs. DMSO group (n=4). HuOCSCs, human ovarian cancer stem cells; Flt-1, fms related tyrosine kinase 1; FVIII, coagulation factor VIII.