Figure 2.

NUDT21 inhibits aggressiveness of bladder cancer cells in vitro. (A) Western blotting analysis of NUDT21 expression in EJ and T24 cells which stably express and silence NUDT21; α-tubulin was used as a loading control. (B) NUDT21 suppresses proliferation in bladder cancer cells, as determined by the CCK-8 assay. (C) Representative micrographs (left panel) and quantification (right panel) of EdU incorporation in bladder cancer cells as indicated. DAPI was used as a DNA/nuclear stain. (D) Mobility of cells was measured with the wound healing assay (left panels); uncovered areas in the wound healing assays were quantified as a percentage of the original wound area (right panels). (E) Representative pictures (left panel) and quantification (right panel) of invading cells as analyzed with a transwell Matrigel assay.