Figure 4.

NUDT21 regulates the length of the 3'-UTR of LIMK2 and ANXA2 genes. (A) Schematic diagram of the 3'-UTR sequences in the model genes. (B) Transcripts with 3'-UTRs of different lengths were identified by real-time qPCR in NUDT21 overexpressing or knockdown cells, and the long transcript-to-total transcript ratios are shown. (C) Abundance of transcripts of different lengths identified by 3'-RACE in each cell line. More transcripts with longer 3'-UTRs were found in NUDT21 overexpressing cells, while more transcripts with shorter 3'-UTRs were found in NUDT21 knockdown cells. (D) Protein levels of LIMK2 and ANXA2 assessed in cells as indicated with western blotting. (E) Efficiency of shRNA targeting of LIMK2 and ANXA2 was evaluated with western blotting. (F) Analysis of TOP/FOP and NF-κB luciferase reporter activity in cells as indicated. (G) Quantification of cells as indicated in the EdU incorporation assay. (H-I) Quantification of invading cells analyzed using wound healing and transwell Matrigel assays. *P<0.05