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. 2019 Sep 23;9(24):7184–7199. doi: 10.7150/thno.38032

Figure 8.

Figure 8

Restored hearing phenotype is unstable. (A) ABR thresholds in response to click sounds were obtained in weekly intervals. Examples from five injected Slc26a4∆/∆ mice that have normal stable hearing (black), stable hearing loss (blue), progressive hearing loss (green), sudden hearing loss (red) and fluctuating hearing loss (cyan). (B) Linear regressions of ABR thresholds in response to click stimuli were obtained in Slc26a4+/+ mice, injected Slc26a4∆/∆ and injected Slc26a4tm1Dontuh/tm1Dontuh mice between 3 and 11 weeks of age. Slopes and Pearson's R value were -0.6 dB/week and R=0.259, 2.9 dB/week and R=0.323, and 4.9 dB/week and 0.391, respectively. (C) ABR thresholds in response to click stimuli were grouped into three age ranges, 3-5 weeks (Slc26a4+/+ n=20, injected Slc26a4∆/∆ n=18, injected Slc26a4tm1Dontuh/tm1Dontuh n=16), 9-11 weeks (Slc26a4+/+ n=17, injected Slc26a4∆/∆ n=17, injected Slc26a4tm1Dontuh/tm1Dontuh n=10), and 16-21 weeks (Slc26a4+/+ n=10, injected Slc26a4∆/∆ n=4, injected Slc26a4tm1Dontuh/tm1Dontuh n=3) to evaluate the stability of hearing in Slc26a4+/+, injected Slc26a4∆/∆ mice and injected Slc26a4tm1Dontuh/tm1Dontuh mice. Data are represented by box-plots (25%, 50%, and 75%) with whiskers (5% and 95%). Outliers were drawn as symbols (diamonds). Differences toward the 3-5 week group were evaluated either by one-way ANOVA with Bonferroni t-test or by Kruskal-Wallis one-way ANOVA on ranks and Dunn's method, n.s. no significant difference, * p<0.05, ** p<0.01, *** p<0.001. (D-F) Fraction of mice that experienced gains or losses (D), losses (E), or gains >15 dB (F) were obtained and differences between injected Slc26a4∆/∆ or injected Slc26a4tm1Dontuh/tm1Dontuh mice and Slc26a4+/+ mice were evaluated by Fisher's exact test, * p<0.05, ** p<0.01, *** p<0.001. Brackets indicate significant differences between injected Slc26a4∆/∆ and injected Slc26a4tm1Dontuh/tm1Dontuh mice, ** p<0.01.