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. 2019 Sep 21;9(24):7108–7121. doi: 10.7150/thno.38182

Figure 6.

Figure 6

RSK-3 knockdown blocks 4-aminobiphenyl (4-ABP)-enhanced cartilage-derived-stem/progenitor cell (CSPC) proliferation and OA protection. (A-B) CSPC proliferation after RSK-3 knockdown (RPS6KA2-shRNA) or RPS6KA2-shRNA plus 4-ABP treatment, determined by CCK-8 assay (A) and Ki67 labeling (B). Nuclei are counterstained with DAPI. Effects of 4-ABP on protein expression of molecules involved in the PI3K-Akt pathway in RPS6KA2-shRNA-treated CSPC (C) and statistical analysis (D). ap < 0.05, bp < 0.01, cp < 0.001 versus vector control. n = 3 per group. Safranin O-Fast green staining of cartilage lesions in RSK-3 knockout mice after destabilization of medial meniscus (DMM) surgery followed by intra-articular 4-ABP (100 μM) injection for 4 weeks (once a week) (E) and OARSI score (F). Scale bars = 100 μm. **p < 0.01 versus sham. n = 5.