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. 2019 Sep 25;9(24):7282–7297. doi: 10.7150/thno.33920

Figure 6.

Figure 6

Sfrp2 overexpression disturbed the protective effect of shSafe on cardiac function post MI. (A) Representative M-mode echocardiographic images obtained from MI mice after Safe knockdown, in combination with Sfrp2 overexpression (n=10 in each group). (B) Sfrp2 overexpression led to a reduced improvement of EF in shSafe-injected MI mice at day 28 post-surgery (n=10). (C) shSafe-mediated increase in FS in MI hearts was inhibited by Sfrp2 overexpression (n=10). (D) Representative images of Masson's trichrome-stained MI hearts, and quantification of relative fibrosis areas (n=8). Scale bar indicates 2 mm. (E) qRT-PCR analysis showing increased expression of Safe, Sfrp2, Col1a1 and α-SMA in left ventricles of shSafe-injected MI hearts after Sfrp2 overexpression (n=3). (F) Western blot analysis and relative densitometric quantification of SFRP2, COL1A1 and α-SMA protein levels in shSafe-injected MI hearts after Sfrp2 overexpression (n=3). All data are presented as mean ± SEM; Student's t-test, one-way ANOVA or two-way repeated-measures ANOVA, *p < 0.05.