Skip to main content
. 2019 Sep 25;9(24):7345–7358. doi: 10.7150/thno.37173

Figure 2.

Figure 2

HAT1/CAF-1 signaling confers to the assembly of HBV cccDNA minichromosome. (A and B) The assembly of histone H3/H4 onto cccDNA was examined by ChIP-qPCR at indicated dpi in HBV-infected dHepaRG cells transfected with siHAT1. (C) The deposition of HBc, HBx and p300 onto cccDNA minichromosome or the promoter of CCNA2 was verified 8 dpi by ChIP-qPCR in HBV-infected dHepaRG cells continuously transfected with siHAT1. (D and E) The assembly of histone H3/H4 onto cccDNA was determined by ChIP-qPCR at indicated dpi in HBV-infected dHepaRG cells continuously transfected with siCAF-1 (p150) (100 nM) at -4, 0, 4 and 8 dpi. (F) The mRNA of CAF-1 were examined by RT-qPCR in the liver of human liver-chimeric mice (n=3) and HBV-infected human liver-chimeric mice (n=3). Mean ± SD of at least three experiments are shown, in which each experiment was designed by three replicates. Statistical significant differences are indicated: **P<0.01; ***P<0.001; ns, no significance.