Figure 7.

The combination of nCUR/nSN38 inhibits the growth of CAC by inducing cell cycle arrest and apoptosis of tumor cells. (A) TUNEL analysis of the excised tumors from each mouse group at the end of the therapeutic studies. (B) Quantification of apoptotic cells by counting TUNEL-positive cells. (C) Immunohistochemical staining of Ki-67 using paraffin-embedded tumor sections and quantification of positive Ki-67 staining. (D) The expression of indicated proteins isolated from the colonic epithelia of healthy mice and tumors of CAC mice after various treatments. The tumors excised from CAC mice without any therapeutic treatments were included as controls. *p < 0.05 and **p < 0.01, as determined by two-way ANOVA followed by Bonferroni's post hoc test.