Figure 2.

MiR-5188 promotes stemness, metastasis, proliferation, and chemoresistance in HCC. (A-E): (A) Hepatosphere formed during three serial passages (scale bar: 20 μm), (B, C) flow cytometry and immunofluorescence (scale bar: 5 μm) analyses, (D) Transwell assays (scale bar: 10 μm) and wound healing assays, and (E) colony formation assays and EdU incorporation assays (Scale bar: 10 μm) of miR-5188-overexpressing Hep3B and Huh7 cells and corresponding control cells. (F) Dose-dependent and time-dependent growth curves of Hep3B and Huh7 cells treated with epirubicin, cisplatin and 5-fluorouracil. Comparison of all groups vs. the control group by Student's t-test, n=3 independent experiments. All data are presented as the mean ± SD. Experiments were repeated three times.