Figure 7.

(A) Multiplexed immunofluorescence staining for PD-L1 (pink), CD11b+ (red), CD45+ (green), CD8+ (yellow) and DAPI (blue) in MC38 tumors, which were measured 4 d (left), 7 d (middle) and 14 d (right) after treatment with saline (control), ¹⁷⁷Lu-EB-RGD targeted radionuclide therapy (TRT), concurrent combined therapy with ¹⁷⁷Lu TRT and αPD-L1 mAb (day 4 and 7) or sequential combined therapy with ¹⁷⁷Lu TRT and αPD-L1 mAb (day 14). Scale bar is 200μm (magnification, × 20). (B-D) Quantified results for multiplexed immunofluorescence staining presented in Figure 7A at different time points. *P < 0.05, **P < 0.01, ***P<0.001.