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Hedgehog (Hh) pathway is activated during CBDL induced liver fibrosis. Relative liver mRNA levels of (A) GLI1, and (B) PTCH1 after treatment with GDC-0449 or MDB5 loaded micelles in CBDL mice. (C) Immunohistochemical (IHC) analysis for protein expression of GLI1 (Objective 20X) and PTCH1 (upper and middle panels) in mouse liver (Objective 10X, inset objective 40X). GLI1 (green) and GLS1 (red) were co-stained with IF (scale bar 100 µm).
