Figure 3.

FADD is a direct target of miR-27a in HEK 293T cells, and apoptosis is partially reduced by FADD inhibition. (A) Schematic representation of the binding between miR-27a and FADD with mutated sites labeled with black line. HUVECs were transfected with an overexpression plasmid for miR-control (NC) or miR-27a and a plasmid encoding luciferase wild-type (WT) FADD 3'UTR or mutated (MT) FADD 3'UTR lacking the miR-27a-binding site. Luciferase activities were determined 24h later (n=3). (B) qRT-PCR and western blotting analysis on the effect of FADD siRNA. (C) Flow cytometer analysis to evaluate HUEVCs apoptosis cotransfected with lentivirus and FADD siRNA (n=5). Data are represented as mean ± SD; *P<0.05.