Table 2.
Sequence (5' to 3') | |
---|---|
Primers | |
hsa-miR-27a-3p | UUCACAGUGGCUAAGUUCCGC |
hsa-miR-27b | UUCACAGUGGCUAAGUUCUGC |
hsa-miR-217 | UACUGCAUCAGGAACUGAUUGGA |
hsa-miR-183 | GUGAAUUACCGAAGGGCCAUAA |
hsa-miR-20a | ACUGCAUUAUGAGCACUUAAAG |
hsa-miR-24 | UGGCUCAGUUCAGCAGGAACAG |
FADD | GGTGGAGAACTGGGATTTGA |
CAACCATCACTGCCCCTACT | |
CASPASE-3 | AAATGGACCTGTTGACCTGAA |
CACAAAGCGACTGGATGAAC | |
CASPASE-8 | TCACAGCATTAGGGACAGGA |
ACTTTGGGTTTTCCAGCAAG | |
TNF-α | CATCTATCTGGGAGGGGTCTT |
GAAGTGGTGGTCTTGTTGCTT | |
BCL-2 | CCCGTTTCCTCTGGTGAAC |
GTGTCTCCGTCCTCATCTGC | |
BAX | ACGACATCAACCGACGCTAT |
GGTGGCAATCTTGGTGAAGT | |
antagomiRs and angomiRs | |
mmu-miR-27a-3p angomiR | UUCACAGUGGCUAAGUUCCGC |
GGAACUUAGCCACUGUGAAUU | |
angomiR NC | UUCUCCGAACGUGUCACGUTT |
ACGUGACACGUUCGGAGAATT | |
mmu-miR-27a-3p antagomiR | GCGGAACUUAGCCACUGUGAA |
antagomiR NC | CAGUACUUUUGUGUAGUACAA |