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. Author manuscript; available in PMC: 2019 Nov 5.
Published in final edited form as: Nat Protoc. 2019 Feb 11;14(3):976–990. doi: 10.1038/s41596-018-0123-5
Reagent Volume Added Final Concentration
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer 25 μl 1X
FWD Primer Set 1 (10μM) 2 μl 0.4 μM
REV Primer Set 1 (10μM) 2 μl 0.4 μM
Template DNA or Overnight Culture of your strain of interest x μl ~ 100 ng
Molecular biology grade H2O Up to 50 μl