Using CAPTURE to detect spacer acquisition in native CRISPR arrays

. Author manuscript; available in PMC: 2019 Nov 5.

Published in final edited form as: Nat Protoc. 2019 Feb 11;14(3):976–990. doi: 10.1038/s41596-018-0123-5

Reagent	Volume Added	Final Concentration
OneTaq® Quick-Load® 2X Master Mix with Standard Buffer	25 μl	1X
FWD Degenerate Primer Master Mix, Set 3 (10μM)	2 μl	0.4 μM
REV Degenerate Primer Set 3 (10μM)	2 μl	0.4 μM
Size Extracted Template DNA	x μl
Molecular biology grade H20	Up to 50 μl