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. 2019 Aug 11;32(4):319–327. doi: 10.1293/tox.2019-0022

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©2019 The Japanese Society of Toxicologic Pathology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/）.

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Fig. 7. — Quantification of the number of acinar cells in the parotid gland. (a, d) Original images. (b, e) Segmented images of acinar cells (yellow) and other regions (pink) using the tissue classifier module. (c, f) Analyzed images of acinar cell size using the vacuole module. Nuclei are blue. Grade of atrophy of acinar cells evaluated by pathologists: (a, c) no change, − (orange); (d, f) slight, + (red). Bar = 100 μm. (g) Quantitative results for acinar cell size. Total area of acinar cells per size. The numbers of acinar cells within 10-μm² size fractions ranging from 0 to 50 μm² and the number of those >50 μm² in size were calculated.