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. 2019 Mar 19;32(4):223–232. doi: 10.1293/tox.2018-0045

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©2019 The Japanese Society of Toxicologic Pathology

This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/）.

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Fig. 2. — DIGE images of differentially expressed protein spots. A, Two-dimensional DIGE images of the control and HFD-induced rats for 0 week, 2 weeks, 4 weeks, 6 weeks, and 8 weeks. B, Protein spots with significant changes were labeled and identified. Spot numbers correspond to those in Supplementary Table 1: online only. pH 3–10, 300 μg proteins were loaded. C, The sequences of the precursor at m/z 1566.84, 1888.04, and 1943.08 were analyzed by MS/MS and found to be ITPSYVAFTPEGER, VTHAVVTVPAYFNDAQR, and DNHLLGTFDLTGIPPAPR. This protein was identified to be the glucose-regulated protein precursor after a database search.