Fig. 6. Customized deployment of SWEET^R lines with the help of the SWEET^R kit 1.0.

Farmers with Xoo-infected rice fields will send samples to local breeders or pathologists, who will isolate respective Xoo strains. Pathologists will then identify induced and critically important SWEET genes using SWEET^up primers for mRNA accumulation and SWEET knock-out (SWEET^KO) mutants. After validation with SWEET EBE-edited Kitaake lines (SWEETp^R), pathologists will identify the optimal SWEET^R line, which is then provided to local breeders. In parallel, labs will isolate Xoo DNA from infected leaves, identify TAL effectors (the TALeome) and predict SWEET targets using SWEETpDB. PathoTracer provides additional region-specific recommendations for deployment of SWEET^R variants.