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. 2019 Oct 30;10:1362. doi: 10.3389/fpls.2019.01362

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Copyright © 2019 He, Zhang, Zhao, Qi, Kang and Guo

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stable genetic transformation of B. distachyon (genotype Bd21-3). (A) Immature embryos grown on callus induction medium; (B) embryo after 3 weeks on callus induction medium; (C) co-cultivation compact embryogenic callus with Agrobacterium on filter paper; (D) co-cultured compact embryogenic callus on selection medium; (E) regenerating callus on regeneration medium; (F) rooted putative transformants on rooting medium; putative transformant grown in soil for 2 weeks (G) and 8 weeks (H), respectively.